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pbabe zeo large t genomic plasmid  (Addgene inc)


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    Addgene inc pbabe zeo large t genomic plasmid
    Pbabe Zeo Large T Genomic Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+zeo+large+t+genomic+plasmid/pBABE-zeo+largeTgenomic+(Plasmid+%231778)/pm41276505-338-1-6
    Average 92 stars, based on 15 article reviews
    pbabe zeo large t genomic plasmid - by Bioz Stars, 2026-10
    92/100 stars

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    Plasmid Preparation:

    Article Title: Targeting fibroblast derived thrombospondin 2 disrupts an immune-exclusionary environment at the tumor front in colorectal cancer.
    Article Snippet: CAFs were cultured in DMEM supplemented with 10% FBS, 1% L-glutamine, and 1% penicillin-streptomycin (Thermo Fisher; 15140-122). .. The pBABE-zeo large T genomic plasmid (Addgene #1778) was introduced into JM109 competent cells (RBC Bioscience; RH717). .. The human embryonic kidney (HEK) 293 T cell line was purchased from the American Type Culture Collection (ATCC, Rockville, USA: catalog number CRL-3216).

    Article Title: Targeting fibroblast derived thrombospondin 2 disrupts an immune-exclusionary environment at the tumor front in colorectal cancer
    Article Snippet: CAFs were cultured in DMEM supplemented with 10% FBS, 1% L-glutamine, and 1% penicillin-streptomycin (Thermo Fisher; 15140-122). .. The pBABE-zeo large T genomic plasmid (Addgene #1778) was introduced into JM109 competent cells (RBC Bioscience; RH717). ..



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    Figure 1. TE expression increases at the subfamily level during transformation. A, Model schematic. Early passage BJ fibroblast cells (EP) were sequentially infected with retrovirus packaged with pBabe-hygro-hTERT (hTERT), <t>pBabe-zeo-SV40</t> large T <t>genomic</t> <t>DNA</t> (LT and ST antigens; SV40), and pBabe-puro-HrasV12 (HRAS). B, Number of significantly differentially expressed TE subfamilies at each stage of transformation (Padj < 0.05, fold change greater than 2 or less than 2). Teal, upregulated elements; violet, downregulated elements. C, Class composition of significantly up- and downregulated elements (Padj < 0.05, fold change greater than 2 or less than 2). Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. D, Overlap of upregulated elements in B between stages of transformation. Yellow, hTERT; red, SV40, blue, HRAS. E, Volcano plot showing log2-fold change between each stage and EP vs. log10 Padj. Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. Gray lines indicate Padj ¼ 0.05 and fold change ¼ 2 and 2. F, Fold change increase in LTR26 by qPCR. Pink, EP; yellow, hTERT; red, SV40; blue, HRAS. Error bars, SEM. Asterisks represent statistically significant differences. , P < 0.05; , P < 0.01. N ¼ 3 for each sample.
    Pbabe Zeo Sv40 Large T Genomic Dna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 1. TE expression increases at the subfamily level during transformation. A, Model schematic. Early passage BJ fibroblast cells (EP) were sequentially infected with retrovirus packaged with pBabe-hygro-hTERT (hTERT), <t>pBabe-zeo-SV40</t> large T <t>genomic</t> <t>DNA</t> (LT and ST antigens; SV40), and pBabe-puro-HrasV12 (HRAS). B, Number of significantly differentially expressed TE subfamilies at each stage of transformation (Padj < 0.05, fold change greater than 2 or less than 2). Teal, upregulated elements; violet, downregulated elements. C, Class composition of significantly up- and downregulated elements (Padj < 0.05, fold change greater than 2 or less than 2). Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. D, Overlap of upregulated elements in B between stages of transformation. Yellow, hTERT; red, SV40, blue, HRAS. E, Volcano plot showing log2-fold change between each stage and EP vs. log10 Padj. Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. Gray lines indicate Padj ¼ 0.05 and fold change ¼ 2 and 2. F, Fold change increase in LTR26 by qPCR. Pink, EP; yellow, hTERT; red, SV40; blue, HRAS. Error bars, SEM. Asterisks represent statistically significant differences. , P < 0.05; , P < 0.01. N ¼ 3 for each sample.
    Pbabe Zeo Large T Genomic, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+zeo+large+t+genomic+plasmid/pBABE-zeo+largeTgenomic+(Plasmid+%231778)/pmc02683118-149-10-14
    Average 92 stars, based on 1 article reviews
    pbabe zeo large t genomic - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 1. TE expression increases at the subfamily level during transformation. A, Model schematic. Early passage BJ fibroblast cells (EP) were sequentially infected with retrovirus packaged with pBabe-hygro-hTERT (hTERT), pBabe-zeo-SV40 large T genomic DNA (LT and ST antigens; SV40), and pBabe-puro-HrasV12 (HRAS). B, Number of significantly differentially expressed TE subfamilies at each stage of transformation (Padj < 0.05, fold change greater than 2 or less than 2). Teal, upregulated elements; violet, downregulated elements. C, Class composition of significantly up- and downregulated elements (Padj < 0.05, fold change greater than 2 or less than 2). Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. D, Overlap of upregulated elements in B between stages of transformation. Yellow, hTERT; red, SV40, blue, HRAS. E, Volcano plot showing log2-fold change between each stage and EP vs. log10 Padj. Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. Gray lines indicate Padj ¼ 0.05 and fold change ¼ 2 and 2. F, Fold change increase in LTR26 by qPCR. Pink, EP; yellow, hTERT; red, SV40; blue, HRAS. Error bars, SEM. Asterisks represent statistically significant differences. , P < 0.05; , P < 0.01. N ¼ 3 for each sample.

    Journal: Cancer Research

    Article Title: Oncogenic Transformation Drives DNA Methylation Loss and Transcriptional Activation at Transposable Element Loci

    doi: 10.1158/0008-5472.can-22-3485

    Figure Lengend Snippet: Figure 1. TE expression increases at the subfamily level during transformation. A, Model schematic. Early passage BJ fibroblast cells (EP) were sequentially infected with retrovirus packaged with pBabe-hygro-hTERT (hTERT), pBabe-zeo-SV40 large T genomic DNA (LT and ST antigens; SV40), and pBabe-puro-HrasV12 (HRAS). B, Number of significantly differentially expressed TE subfamilies at each stage of transformation (Padj < 0.05, fold change greater than 2 or less than 2). Teal, upregulated elements; violet, downregulated elements. C, Class composition of significantly up- and downregulated elements (Padj < 0.05, fold change greater than 2 or less than 2). Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. D, Overlap of upregulated elements in B between stages of transformation. Yellow, hTERT; red, SV40, blue, HRAS. E, Volcano plot showing log2-fold change between each stage and EP vs. log10 Padj. Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. Gray lines indicate Padj ¼ 0.05 and fold change ¼ 2 and 2. F, Fold change increase in LTR26 by qPCR. Pink, EP; yellow, hTERT; red, SV40; blue, HRAS. Error bars, SEM. Asterisks represent statistically significant differences. , P < 0.05; , P < 0.01. N ¼ 3 for each sample.

    Article Snippet: Briefly, early passage BJ fibroblast cells (EP) were sequentially infected with retrovirus packaged with pBabe-hygro-hTERT (RRID:Addgene_1773), pBabe-zeo-SV40 large T genomic DNA (LT and ST antigens; RRID:Addgene_1778) and pBabe-puroHrasV12 (RRID:Addgene_1768).

    Techniques: Expressing, Transformation Assay, Infection